In the 5 years since its launch, VISICORT has gathered a unique collection of biological samples from corneal transplant recipients across 5 European countries. We are especially motivated to ensure that the generosity of these patients has a legacy of new research that continues to grow beyond the life-time of the project itself. Lisa’s exciting PhD project is a perfect example of this potential. I am particularly proud that we have been able to attract a talented scientist at the University of Edinburgh into the field of corneal transplant biology and that her research has the potential to directly benefit patients with corneal disease in the future.VISICORT Coordinator, Professor Matthew Griffin
Lisa Imrie is a Proteomics Specialist at the University of Edinburgh and a graduate of Edinburgh Napier University with a degree in microbiology and biotechnology. She has recently started her PhD studies taking a unique perspective in the analysis of biosamples from the VISICORT Foundation Biobank.
In this interview (2 of 2), Danielle Nicholson, Pintail Limited poses questions to Lisa about her work in proteomics, what a career in laboratory science really looks like and her PhD research.
What is proteomics and how did this become an interest for you?
Proteomics is the large-scale study of proteomes (a set of proteins produced by an organism/system). It can be used to investigate when/where proteins are expressed, protein production rates, protein modifications (e.g. PTMs), protein-protein interactions, and how proteins are involved in metabolic pathways, amongst other things.
I first became interested in proteomics between the third and fourth year of my undergraduate degree. I worked for the summer in a local research institute in what was then their “Functional Genomics Unit” which had one mass spectrometer which I used to identify proteins that were present in researcher’s samples by peptide mass fingerprinting. I was then invited back to complete my honours project within the same unit and that’s when I was hooked. As luck would have it, on the completion of my honours project a position came up within the expanding unit that I successfully applied for and thus began my career in mass spectrometry and proteomics.
What are your main goals as a PhD researcher?
One main goal of this project is to identify clinically useful biomarkers in eye disease patients. To this end, I will use mass spectrometry instrumentation to identify proteomic, metabolomic and lipidomic markers in different eye tissues across both keratoconus and FECD. This will be the first time in ophthalmic research that this combination of omics data sets will be integrated to provide a better understanding of the disease mechanisms of these corneal diseases.
Another goal is to build as wide a knowledge base as possible around my chosen topic. Every day I’m learning more and more and each new fact will undoubtedly lead to more research down a different avenue. Compiling all this information to give me the best chance of successfully completing this project will be an ongoing effort.
“I love immersing myself in the literature related to this topic, applying it to my own work and theories and seeing what direction my research will take me in on a daily basis!”Lisa Imrie, PhD candidate at the University of Edinburgh
Give us a snapshot of your work environment and day.
I work in the Kings Buildings campus of the University of Edinburgh. There are many campuses across the city and we’re based on the south side. All of Science and Engineering (including the School of Biological Sciences where I work) are based on this campus. The facility I work in is called EdinOmics and there are currently four staff members who work here: an academic leader, a metabolomics specialist, a proteomics specialist (me) and a lab manager. Our mass spectrometry lab is situated on the ground floor and we all have offices dotted about the building. I have an office just opposite the lab which is great for popping in and checking on the status of all the instruments and generally keeping an eye on things. For all sample preparation, we have a wet lab on the first floor which has all the general lab equipment you’d expect to see in any biological lab. Within our building, there are six different research groups and we all share the same wet lab, however, our mass spectrometry lab is just dedicated to the EdinOmics group and the instruments.
Describe your typical day. What do you do? What skills do you need?
A normal day for me would start with arriving at work at around 8.30 and heading straight to the mass spectrometry lab. The first thing I like to do before anything else is to check that the instruments are working as expected and there are no blinky red lights on anything – not good news! If there have been samples run the previous day or through the night I’ll start the data analysis for them which will involve the files being converted into a database searchable format which can take a little time. Whilst this is ticking over I’ll head to the office and switch on my PC before making myself a cup of coffee. My day will then consist of any combination of:
· Data analysis from samples run the previous day/overnight
· Reporting of results to clients either by email or in person
· Meeting with potential clients who are interested in using the facility to answer their research questions
· Any general lab maintenance requiring attention
Throughout all this, whenever I have time, I will focus on my own PhD project. At the moment this will include the ongoing literature search to broaden my knowledge of the topic and keep up to date with what’s been achieved in the field so far as well as planning any pilot experiments to work up the methods I plan to use throughout my project. All this juggling requires a lot of time management skill and it is definitely one of the most difficult parts of my job. Finding time for both running the facility and my PhD project can be challenging!
What are your main interests outside science?
I have a young family (11yr old daughter and 7yr old son) so my interests outside of work revolve mainly around them! They definitely have better social lives than I do and between my husband and myself, we spend most of our time taxiing them around to various clubs/sports. It’s great to see them grow into confident young people so I wouldn’t have it any other way. If I manage to get a minute to myself I like to socialise with family and friends and put the world to rights over dinner & drinks.
What advice would you give to a young person wanting to pursue a career in science?
Keep an open mind and don’t try to “specialise” too early. I went from an interest in marine biology to microbiology to biotechnology and ended up working with mass spectrometers in the omics fields. Similarly, don’t be scared to branch out even when you have decided on your field of interest. If someone had told me a couple of years ago that I’d be doing a PhD related to ophthalmology I would have laughed at them. It’s amazing where a career in science can take you!
Sum up what you find most interesting about your work now in just one sentence.
I love immersing myself in the literature related to this topic, applying it to my own work and theories and seeing what direction my research will take me in on a daily basis!